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Molecular Plant Virology |
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Dr. S. K. Raj
Scientist G
| Phone no. 91-522-2205831-35 Ext. 950 | sk.raj@nbri.res.in |
Dr. J. A. Khan
Scientist E I
| Phone no. 91-522-2205831-35 Ext. 951 | jawaidkhan@nbri.res.in |
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Group works on
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- Biological, biochemical and molecular characterization of plant viruses affecting
ornamental, horticultural and crop plant.
- Development of diagnostics for plant viruses for their sensitive detection in economically
important crops.
- Development of transgenic plants for resistance against viruses.
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Objectives
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- Authentic identification of virus isolates for placing them in a proper group/ subgroup.
- Development of sensitive diagnostic kits for virus indexing of Indian isolates of
Cucumoviruses and Geminiviruses in commercial crops.
- Genetic transformation of tobacco, chilli and tomato for raising of virus resistant
transgenic plants utilizing CP and Replicase gene of Tomato Leaf Curl Virus and
Cucumber mosaic virus.
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Achievements
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National
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- Plant viruses viz. Eggplant mottled crinkle virus (EMCV) from Solanim melongena
& S. capsicastrum, Chrysanthemum aspermy virus (CAV) from Chrysanthemum
and Cucumber mosaic virus (CMV) from Chrysanthemum, Carnation, Amaranth, Datura,
Gladiolus, Banana, Tomato and Henbane have been characterized and their antisera
have been raised.
- Immuno-detection techniques viz. ELISA, ISEM, Western blot assay have been standardized
for detection of EMCV, CMV and CAV. PCR for Tomato leaf curl virus in tomato, cucurbits,
croton & weeds and RT-PCR for Cucumber mosaic virus in Amaranth, Chrysanthemum,
Gladiolus, Banana, Tomato & Datura have been developed for sensitive diagnosis.
- Coat protein and movement protein Cucumber mosaic virus (Amaranth) and DNA-A of
Pumpkin yellow mosaic virus were cloned and sequenced for molecular characterization.
- Transformation of tomato using coat protein gene of CMV and Tomato leaf curl virus
has been done and transgenic plants expressing coat protein of CMV and TLCV have
been successfully generated.
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International
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- An isolate of Eggplant Mottled Crinkle Tombusvirus from Solanum melongena
and its polyclonal antibody deposited to the American Type Culture Collections,
USA.
- Sequence data of coat protein gene of CMV-Amaranth (AF 198622), CMV-Datura (AF 281864),
CMV-Henbane (AF 350450), movement protein of CMV-A (AF 414078), coat protein region
of TLCV-Pumpkin (AF 381977, AY 396151), Acalypha yellow mosaic virus (AY 188956),
Indian Croton gemini virus (AF 188965) and Tomato yellow leaf curl virus (AF 375241)
submitted to GenBank.
- Collaboration with Scottish Crop Research Institute, Dundee, UK for research on
viruses associated with Groundnut Rosette disease of groundnut.
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Research Publications: 75
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National journals
International
Reviews in Book
Proceedings
TV talk
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: 35
: 30
: 03
: 05
: 02
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Feedback required
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- Groups working in this area in India as well as in abroad are welcome for academic
exchange.
- Industrial needs for commercialization of virus diagnostic kits developed by the
group.
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